Media - LB (Luria-Bertani)
by A. Untergasser (contact address and download at
Version: 1.0 - Print Version (.PDF)
This is a very basic recipe used in any lab.
It is probably THE most used bacteria medium.
Costs: 0.85 euro/liter
|--||add 950 ml water|
|adjust the pH to 7.0 with 5 M NaOH|
|--||add water to 1 liter|
Autoclave for 20 min at 120 °C
Let cool to ca. 55 °C before adding antibiotics
Store at room temperature or 4 °C
Prepare LB as described above, but add before autoclaving:
|1,5 % Agar||15 g Agar|
Tryptone (# T1332.1000) by Duchefa
Yeast Extract (# Y1333.1000) by Duchefa
- I always use LB with half of the NaCl concentration and I have no clue why.
- I never ajusted the pH because I am lazy and the bacteria didn't mind it.
References and Comments:
This is a known recipe as it can be found for example in "Molecular Cloning: A Laboratory Manual".
How to cite this page in publications:
This document can be cited like this:
Untergasser A. “Media - LB (Luria-Bertani)” Untergasser's Lab. Summer 2006 (include here the date when you accessed these page).
Please Do Not Reprint This Article:
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